Lab Experiments

OB-fold nucleic acid binding domain-containing protein

DEAD/DEAH box helicase domain-containing protein

OB-fold nucleic acid binding domain-containing protein

Minichromosome maintenance protein MCM

OB-fold nucleic acid binding domain-containing protein

DNA mismatch repair protein MutS; This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity

OB-fold nucleic acid binding domain-containing protein

DNA polymerase sliding clamp; Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication

OB-fold nucleic acid binding domain-containing protein

DNA polymerase 2

OB-fold nucleic acid binding domain-containing protein

DNA primase, large subunit; Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair

OB-fold nucleic acid binding domain-containing protein

DNA primase small subunit; Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. [...]

OB-fold nucleic acid binding domain-containing protein

DNA repair and recombination protein RadA; Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules

OB-fold nucleic acid binding domain-containing protein

DNA repair and recombination protein RadB; Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange

DEAD/DEAH box helicase domain-containing protein

OB-fold nucleic acid binding domain-containing protein

DEAD/DEAH box helicase domain-containing protein

DNA mismatch repair protein MutS; This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity

DEAD/DEAH box helicase domain-containing protein

DNA polymerase sliding clamp; Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication

DEAD/DEAH box helicase domain-containing protein

DNA polymerase 2

DEAD/DEAH box helicase domain-containing protein

DNA repair and recombination protein RadA; Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules

DEAD/DEAH box helicase domain-containing protein

DNA repair and recombination protein RadB; Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange

DEAD/DEAH box helicase domain-containing protein

Single-stranded DNA-binding replication protein A

DEAD/DEAH box helicase domain-containing protein

DNA topoisomerase VI subunit A; Relaxes both positive and negative superturns and exhibits a strong decatenase activity

Toprim domain-containing protein

tRNA-splicing endonuclease; Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5’ and 3’ splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2’,3’ cyclic phosphate and 5’-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp

Toprim domain-containing protein

DNA mismatch repair protein MutS; This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity

Toprim domain-containing protein

DNA polymerase 2